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The Ag+ mild and PEI have medium cytotoxic effects, respectively. All antimicrobial agents have some degree of cytotoxic effect. The degree of cytotoxicity depends on the sensitivity of the microbial species, the characteristics of the environment, and in our study, the modification of agents, notably PEI. The cytotoxicity of PEI depends on the number of reactive amino groups at the free terminals of the chains . Forming complexes with Ag+, the number of free PEI amino groups will decrease. Our comparative evaluation of the extracts revealed that the Ag-PEI-PLA extract had significantly less cytotoxic effects than the derivative PEI-PLA extract containing no silver. The time course evaluation of nanoparticle release (day 1, day 5, and day 10) also demonstrated that over a long period, the Ag-PEI-PLA extract is more advantageous, as it has less apoptosis induction ability. To summarise, the cell biology analysis data show that the nanosilver-containing novel composite (Ag-PEI-PLA) is better tolerated by the cells of the superficial layers of the oral cavity.
The incidence of all forms of candidiasis have increased in recent decades. This is due to developments in medicine, with more invasive medical procedures and surgeries, more widespread use of broad spectrum antibiotics and immunosuppression therapies. The HIV/AIDs global pandemic has been the greatest factor in the increased incidence of oral candidiasis since the 1980s. The incidence of candidiasis caused by NCAC species is also increasing, again thought to be due to changes in medical practise (e.g., organ transplantation and use of indwelling catheters). 153554b96e